RNA
LSLRγ
Part:BBa_K2841543
Designed by: Jack Lawrence, Rhys Evans, Jonny Whiteside Group: iGEM18_Warwick (2018-10-13)
Legionella Sensing LacI Repressing gRNA - Gamma
LSLR gRNA Gamma: A dCAS9 (BBa_K2841510) gRNA (BBa_K2841514) capable of repressing the LacIq promoter (BBa_K2841517) in response to [http://2018.igem.org/Team:Warwick/Legiolysin Legionella Hemolysin] encoding mRNA. Recognises the Gamma (BBa_K2841533) imitating motif.
This part shows 1.8-fold upregulation upon induction of activity.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 152
Illegal NgoMIV site found at 181 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |